2010:



Schmidt S, Essmann F, Cirstea IC, Kuck F, Thakur HC, Singh M, Kletke A, Jänicke RU, Wiek C, Hanenberg H, Ahmadian MR, Schulze-Osthoff K, Nürnberg B, Piekorz RP. The centrosome and mitotic spindle apparatus in cancer and senescence. Cell Cycle. 2010 Nov 20;9(22). [Epub ahead of print]


Abstract


Schmidt S, Schneider L, Essmann F, Cirstea IC, Kuck F, Kletke A, Jänicke RU, Wiek C, Hanenberg H, Ahmadian MR, Schulze-Osthoff K, Nürnberg B, Piekorz RP. The centrosomal protein TACC3 controls paclitaxel sensitivity by modulating a premature senescence program. Oncogene. 2010 Aug 23. [Epub ahead of print]


Abstract



Scholz RP, Gustafsson JO, Hoffmann P, Jaiswal M, Ahmadian MR, Eisler S, Erlmann P, Schmid S, Hausser A, Olayioye MA. The tumor suppressor protein DLC1 is regulated by PKD-mediated GAP domain phosphorylation. Exp Cell Res. 2010 Nov 15. [Epub ahead of print]



Abstract


Frasa MA, Maximiano FC, Smolarczyk K, Francis RE, Betson ME, Lozano E, Goldenring J, Seabra MC, Rak A, Ahmadian MR, Braga VM. Armus is a Rac1 effector that inactivates Rab7 and regulates E-cadherin
degradation. Curr Biol. 2010 Feb 9;20(3):198-208.


Abstract



Lothar Gremer*, Torsten Merbitz-Zahradnik*, Radovan Dvorsky*, Ion C. Cirstea*, Christian Peter Kratz, Martin Zenker, Alfred Wittinghofer, Mohammad Reza Ahmadian. Germline KRAS mutations cause aberrant biochemical and physical properties leading to developmental disorders. Hum Mutat. 2010 Oct 14. [Epub ahead of print]. *, equal contribution.



Abstract


Gazdag EM, Cirstea IC, Breitling R, Lukes J, Blankenfeldt W and Alexandrov K. Purification and crystallization of human Cu/Zn superoxide dismutase recombinantly produced in the protozoan Leishmania tarentolae. Acta Cryst. (August 2010). F66, 871–877.

Abstract



Chen YX, Koch S, Uhlenbrock K, Weise K, Das D, Gremer L, Brunsveld L, Wittinghofer A, Winter R, Triola G, Waldmann H. Synthesis of the Rheb and K-Ras4B GTPases. Angew Chem Int Ed Engl. 2010 Jul 22.

Abstract



Rosnizeck IC, Graf T, Spoerner M, Tränkle J, Filchtinski D, Herrmann C, Gremer L, Vetter IR, Wittinghofer A, König B, Kalbitzer HR. Stabilizing a weak binding state for effectors in the human ras protein by cyclen complexes. Angew Chem Int Ed Engl. 2010 May 17;49(22):3830-3.

Abstract



Gremer L, De Luca A, Merbitz-Zahradnik T, Dallapiccola B, Morlot S, Tartaglia M, Kutsche K, Ahmadian MR, Rosenberger G. Duplication of Glu37 in the switch I region of HRAS impairs effector/GAP binding and underlies Costello syndrome by promoting enhanced growth factor-dependent MAPK and AKT activation. Hum Mol Genet. 2010 Mar 1;19(5):790-802.

Abstract



Frasa MA, Maximiano FC, Smolarczyk K, Francis RE, Betson ME, Lozano E, Goldenring J, Seabra MC, Rak A, Ahmadian MR, Braga VM. Armus is a Rac1 effector that inactivates Rab7 and regulates E-cadherin degradation. Curr Biol. 2010 Feb 9;20(3):198-208.

Abstract



Cirstea IC, Kutsche K, Dvorsky R, Gremer L, Carta C, Horn D, Roberts AE, Lepri F, Merbitz-Zahradnik T, König R, Kratz CP, Pantaleoni F, Dentici ML, Joshi VA, Kucherlapati RS, Mazzanti L, Mundlos S, Patton MA, Silengo MC, Rossi C, Zampino G, Digilio C, Stuppia L, Seemanova E, Pennacchio LA, Gelb BD, Dallapiccola B, Wittinghofer A, Ahmadian MR, Tartaglia M, Zenker M. A restricted spectrum of NRAS mutations causes Noonan syndrome. Nat Genet. 2010 Jan;42(1):27-9.

Abstract




                                                                                        2009:




Kurig B, Shymanets A, Bohnacker T, Prajwal, Brock C, Ahmadian MR, Schaefer M, Gohla A, Harteneck C, Wymann MP, Jeanclos E, Nürnberg B.

Proc Natl Acad Sci U S A. 2009 Dec 1;106(48):20312-7. Epub 2009 Nov 11.PMID: 19906996 [PubMed - in process]Related articles


Eberth, A. and Ahmadian, M.R. In vitro GEF and GAP assays. Curr. Protoc. Cell Biol., 2009 Jun;Chapter
14:Unit 14.9.


Shymanets, A., Ahmadian, M.R. and Nürnberg, B. (2009) Gβγ-copurified lipid kinase impurity from Sf9 cells. Protein Pept Lett. 2009 Sep 10. [Epub ahead of print].


Eberth, A., Lundmark, R., Gremer, L., Dvorsky, R., Koessmeier, K.T., McMahon, H.T. and Ahmadian, M.R. (2009) A BAR domain-mediated autoinhibitory mechanism for RhoGAPs of the GRAF family. Biochem. J. 417, 371-377.


2008:


Gremer, L., Gilsbach, B., Ahmadian, M.R. and Wittinghofer, A. (2008) Fluoride complexes of oncogenic Ras mutants to study the Ras-RasGAP-interaction. Biol. Chem. 389, 1163-1171.

Gasper, R., Thomas, C., Ahmadian, M.R. and Wittinghofer, A. (2008) The role of the conserved switch II glutamate in guanine nucleotide exchange factor-mediated nucleotide exchange of GTP-binding proteins. J. Mol. Biol. 379, 51-63.

Pena, V., Hothorn, M., Eberth, A., Kaschau, N., Parret, A., Gremer, L., Bonneau, F., Ahmadian, M.R. and Scheffzek, K. (2008) The C2 domain of synGAP is essential for stimulation of the Rap GTPase reaction. EMBO Rep., 9, 344–349.


2007:


van der Burgt, I., Kupsky, W., Stassou, S., Nadroo, A., Barroso, C., Diem, A., Kratz, C., Dvorsky, R., Ahmadian, M.R. and Zenker, M. Myopathy caused by HRAS germline mutations - implications on disturbed myogenic differentiation in the presence of constitutive H-Ras activation. J.Med.Genet., 44, 459-462.

Olsen, H., Herbrand, U., ter Veld, F., Ahmadian, M.R. and Wehner, F. (2007) Differential regulation of cell volume and shape in confluent rat hepatocytes under hypertonic stress. Cell. Physiol. Biochem. 19, 259-268.

Roeglin, L., Hemsath, L., Ahmadian, M.R. and Seitz, O. (2007) DNA-triggered reversible switching of peptide conformation and bioactivity. Angew. Chem. Int. Ed. Engl. 46, 2704-2707.

Gotthardt, K. and Ahmadian, M.R. (2007) Asef is a Cdc42-specific guanine nucleotide exchange factor. Biol. Chem., 388, 67-71.


2006:


Haeusler, L.C, Hemsath, L., Fiegen, D., Blumenstein, L., Herbrand, U., Stege, P., Dvorsky, R. and Ahmadian, M.R. (2006) Purification and biochemical properties of Rac 1,2,3 and the splice variant Rac1b. Methods Enzymol. 406, 1-11.

Herbrand, U. and Ahmadian, M.R. (2006) p190RhoGAP as an integral component of the Tiam1/Rac1 induced downregulation of Rho. Biol. Chem., 387, 311-317.

Fiegen, D., Dvorsky, R. and Ahmadian, M.R. Structural principles of Ras interaction with regulators and effectors. In: Proteins and Cell Regulation, Kluwer Academic Publishers, Dordrecht, The Netherlands (C.J. Der, editor), 4, 45-66.


2005:


Hemsath, L., Dvorsky, R., Fiegen, D., Carlier, M.F. and Ahmadian, M.R. (2005) An Electrostatic Steering Mechanism of Cdc42 Recognition by Wiskott-Aldrich Syndrome Proteins. Mol. Cell 20, 313-324.

Rose, R., Weyand, M., Lammers, M., Ishizaki, T, Ahmadian, M.R. and Wittinghofer, A. (2005) Structural and mechanistic insights into the Rho•mDia interaction. Nature. 435, 513-518.

Scheffzek, K. and Ahmadian, M.R. (2005) GTPase activating proteins – structural and functional insights 18 years after discovery. Cell. Mol. Life Sci., 62, 3014-3038.

Guo, Z., Ahmadian, M.R. and Goody, R.S. (2005) Guanine nucleotide exchange factors operate by a simple allosteric competitive mechanism. Biochemistry 44, 15423-15429.

Hemsath, L. and Ahmadian, M.R. (2005) Fluorescence approaches for monitoring interactions of RhoGTPases with nucleotides, regulators and effectors. Methods 37, 173–182.

Eberth, A., Dvorsky, R., Becker, C., Beste, A., Goody, R.S. and Ahmadian, M.R. (2005) Monitoring the real-time kinetics of the hydrolysis reaction of guanine nucleotide binding proteins. Biol. Chem. 386, 1105–1114.


2004:


Dvorsky, R. and Ahmadian, M.R. (2004) Always look on the bright site of Rho-structural implications for a conserved intermolecular interface. EMBO Rep. 5, 1130-1136.

Blumenstein, L. and Ahmadian, M.R. (2004) Models of the cooperative mechanism for Rho-effector recognition: Implications for RhoA-mediated effector activation. J. Biol. Chem. 279, 53419-53426.

Blochl, A., Blumenstein, L. and Ahmadian, M.R. (2004) Inactivation and activation of Ras by the neurotrophin receptor p75. Eur. J. Neurosci., 20, 2321-2335.

Uhlenbrock, K., Eberth, A., Herbrand, U., Daryab, N., Stege, P., Meier, F., Friedl, P., Collard, J. and Ahmadian, M.R. The RacGEF Tiam1 inhibits migration and invasion of metastatic melanoma via a novel adhesive mechanism. J. Cell Sci. 117, 4863-4871.

Fiegen, D., Haeusler, L.C., Blumenstein, L., Herbrand, U., Dvorsky, R., Vetter, I.R. and Ahmadian, M.R. (2004) Alternative splicing of Rac1 creates a self-activating GTPase. J. Biol. Chem. 279, 4743-4749.

Dvorsky, R., Blumenstein, L., Vetter, I.R. and Ahmadian, M.R. (2004) Structural insights into the interaction of ROCKI with the switch regions of RhoA. J. Biol. Chem. 279, 7098-7104.


2003:


Fauchereau, F., Herbrand, U., Chafey, P., Eberth, A., Koulakoff, A., Vinet, M.C., Ahmadian, M.R., Chelly, J.,and Billuart, P. (2003) The RhoGAP activity of OPHN1, a new F-actin binding protein, is negatively controlled by its amino-terminal domain. Mol. Cell. Neurosci. 23, 574-586.

Ahmadian, M.R., Kiel, C., Stege, P. and Scheffzek, K. (2003) Structural Fingerprints of the Ras-GTPase Activating Proteins Neurofibromin and p120GAP. J. Mol. Biol. 329, 699–710.

Tiede, I., Fritz, G., Strand, S., Poppe, D., Dvorsky, R., Strand, D., Lehr, H.A., Wirtz, S., Becker, C., Atreya, R., Mudter, J., Hildner, K., Bartsch, B., Holtmann, M., Blumberg, R., Walczak, H., Iven, H., Galle, P.R., Ahmadian, M.R., Neurath, M.F. (2003) CD28-dependent Rac1 activation is the molecular target of azathioprine in primary human CD4+ T lymphocytes. J. Clin. Invest. 111, 1133-1145.

Oliveira, M.J., Van Damme, J., Lauwaet, T., De Corte, V., De Bruyne, G., Verschraegen, g., Vaneechoutte, M., Goethals, M., Ahmadian, M.R., Müller, O., Vandekerckhove, J. Mareel, M. and Leroy, A. (2003) b-Casein-Derived Peptides, Produced by Bacteria, Stimulate Cancer Cell Invasion and Motility. EMBO J. 22, 6161-6173.

Haeusler, L.C., Blumenstein, L., Stege, P., Dvorsky, R. and Ahmadian, M.R. (2003) Comparative functional analysis of the Rac GTPases. FEBS Lett. 555, 556-560.


2002:


Fiegen, D., Blumenstein, L., Stege P., Vetter, I.R. and Ahmadian, M.R. (2002) Crystal structure of Rnd3/RhoE: functional implications. FEBS Lett. 525, 100-104.

Ahmadian, M.R., Wittinghofer, A. and Herrmann, C. (2002) Fluorescence Methods in the Study of Small GTP-Binding Proteins. Methods Mol. Biol. 189, 45-63.

Brinkmann, T., Daumke, O., Herbrand, U., Kuhlmann, D., Stege, P., Ahmadian, M.R. and Wittinghofer, A. (2002) Rap-specific GTPase activating protein follows an alternative mechanism. J. Biol. Chem. 277, 12525-12531.

Ahmadian, M.R. (2002) Prospects for anti-Ras drugs. Brit. J. Haematol. 116, 511-519. Karaguni, I.M., Herter, P., Debruyne, P., Chtarbova, S., Kasprzynski, A., Herbrand, U., Ahmadian, M.R., Glusenkamp, K.H., Winde, G., Mareel, M., Moroy, T., and Muller, O. (2002) The new Sulindac derivative IND 12 reverses Ras-induced cell transformation. Cancer Res. 62, 1718-1723.

Ahmadian, M.R., Wittinghofer, A. and Schmidt, G. (2002) The actin filament architecture: tightly regulated by the cells, manipulated by pathogens: International Titisee Conference on the actin cytoskeleton: from signaling to bacterial pathogenesis. EMBO Rep. 3, 214-218.

Ahmadian, M.R., Wittinghofer, A. and Schmidt, G. (2002) The actin filament architecture: tightly regulated by the cells, manipulated by pathogens: from signaling to bacterial pathogenesis. B.I.F. Futura 17, 5-12.


2001:


van Horck, F.P.G., Ahmadian, M.R., Haeusler, L.C., Moolenaar, W.H. and Kranenburg, O. (2001) Characterisation of p190RhoGEF: a RhoA-specific Guanine Nucleotide Exchange Factor that interacts with microtubules. J. Biol. Chem. 276, 4948-4955.

Würtele, M., Wolf, E., Pederson, K.J., Ahmadian, M.R., Barbieri, J.T. and Wittinghofer, A. (2001) Downregulation of GTP-binding protein by a bacterial toxin at high resolution. Nat. Struct. Biol. 8, 23-26.

Allin, C., Ahmadian, M.R., Wittinghofer, A. and Gerwert, K. (2001) Monitoring the GAP Catalyzed H-Ras p21 GTPase Reaction at atomic resolution in real time. Proc. Natl. Acad. Sci. USA 98, 7754-7759.

Demunter, A., Ahmadian, M.R., Libbrecht, L., Stas, M., Baens, M., Scheffzek, K.,Degreef, H., De Wolf-Peeters, C. and van den Oord, J.J.(2001) A novel N-ras mutation in malignant melanoma is associated with excellent prognosis. Cancer Res. 61, 4916-4922.

Gail, R., Costinella, B., Ahmadian, M.R. and Wittinghofer, A. (2001) Ras-mediated cleavage of a GTP analogue by a novel mechanism. Chembiochem 2, 570-575.


2000:


Klockow, B., Ahmadian, M.R., Block, B. and Wittinghofer, A. (2000) Oncogenic insertional mutations in the P-loop of Ras are overactive in MAP kinase signaling. Oncogene 19, 5367-5376.


1999:


Ahmadian, M.R., Zor, T., Vogt, D., Kabsch, W., Selinger, Z., Wittinghofer, A. and Scheffzek, K. (1999) Guanosine triphosphatase stimulation of oncogenic Ras mutants. Proc. Natl. Acad. Sci. USA 96, 7065-7070.

Reid, T., Bathoorn, A, Ahmadian, M.R.  and Collard, J.G. (1999) Identification and characterization of hPEM-2, a guanine nucleotide exchange factor specific for Cdc42. J. Biol. Chem. 274, 33587-33593.

Ostermann, N., Ahmadian, M.R., Goody, R.S. and Wittinghofer, A. (1999) New N-2-labelled fluorescent derivatives of guanine nucleotides and their interaction with GTP-binding proteins. Nucleos. Nucleot. 18, 245-262.

Scheffzek, K., Ahmadian, M.R., Wiesmuller, L., Lautwein, A., Kabsch, W., Schmitz, F. and Wittinghofer, A. (1999) The Ras-RasGAP complex: How to complement an inefficient active site. Phosph. Sulfur Silicon Rel Elem. 146, 741-744.


1998:


Scheffzek, K., Ahmadian, M.R., Wiesmüller, L., Kabsch, W., Stege, P., Schmitz, F. and Wittinghofer, A. (1998) Structural analysis of the GAP related domain from neurofibromin and its implications. EMBO J. 17, 4313-4327.

Scheffzek, K., Ahmadian, M.R. and Wittinghofer, A.(1998) GTPase-activating proteins: helping hands to complement an active site. Trends Biochem. Sci.23, 257-262.

Herrmann, C., Ahmadian, M.R., Hofmann, F. and Just, I. (1998) Functional Consequences of Monoglucosylation of Ha-Ras at Effector Domain Amino Acid Threonine 35. J. Biol. Chem. 273, 16134-16139.

Ahmadian, M.R., Scheffzek, K. and Wittinghofer, A.(1998) Are all GTP-binding proteins switched off by arginine fingers? Trends Biochem. Sci. 23, 260.

Klose, A., Ahmadian, M.R., Schuelke, M., Scheffzek, K., Hoffmeyer, S., Gewies, A., Schmitz, F., Kaufmann, D., Peters, H., Wittinghofer, A. and Nürnberg, P. (1998) Selective disactivation of neurofibromin GAP activity in neurofibromatosis type 1 (NF1). Human Mol. Genet. 7, 1261-1268.


1997:


Ahmadian, M.R., Stege, P., Scheffzek, K. and Wittinghofer, A. (1997) Confirmation of the arginine-finger hypothesis for the GAP-stimulated GTP-hydrolysis reaction of Ras. Nat. Struc. Biol. 4, 686-689.

Scheffzek, K., Ahmadian, M.R., Kabsch, W., Wiesmüller, L., Lautwein, A., Schmitz, F. and Wittinghofer, A.(1997) The Ras-RasGAP complex: structural basis for GTPase activation and its loss in oncogenic Ras mutants. Science 277, 333-338.

Ahmadian, M.R., Hoffmann, U., Goody, R.S. and Wittinghofer, A. (1997) Individual Rate Constants for the Interaction of Ras Proteins with GTPase-Activating Proteins Determined by Fluorescence Spectroscopy. Biochemistry 36, 4535-4541.

Wittinghofer, A., Scheffzek, K. and Ahmadian, M.R. (1997) The Interaction of Ras with GTPase-Activating Proteins. FEBS Lett. 410, 63-67.

Ahmadian, M.R., Mittal, R., Hall, A. and Wittinghofer, A. (1997) Formation of a transition state analogue of the GTPase reaction between small GTP-binding proteins and their GAPs. FEBS Lett. 408, 315-318.


1996:


Mittal, R., Ahmadian, M.R., Goody, R.S. and Wittinghofer, A. (1996) Formation of a Transition-State Analog of the Ras GTPase Reaction by Ras•GDP, Tetrafluoroaluminate and GAPs. Science273, 115-117.

Scheffzek, K., Lautwein, A., Kabsch, W., Ahmadian, M.R.  and Wittinghofer, A. (1996) 3-dimensional structure of the GTPase-activating domain of the human p120GAP: implications for the interaction with Ras. Nature 284, 591-596.

Ahmadian, M.R., Wiesmuller, L., Lautwein, A. and Wittinghofer, A. (1996) Structural Differences in the Minimal Catalytic Domains of the GTPase-Activating Proteins p120GAP and Neurofibromin. J. Biol. Chem.27
16409-16415.


1995:


Nock, S., Grillenbeck, N., Ahmadian, M.R., Ribeiro, S., Kreuzer, R. and Sprinzl M. (1995) Properties of isolated domains of the elongation factor Tu from Thermus thermophilus HB8. Eur. J. Biochem. 234, 132-139.

Ahmadian, M.R., Kreuzer, R., Blechschmidt, B. and Sprinzl M. (1995) Site-directed mutagenesis of Thermus thermophilus EF-Tu: the substitution of threonine-62 by serine or alanine. FEBS Lett. 377, 253-257.


1993:


Ahmadian, M.R., Ghozlan, H.A., Sabry, S. and Kleiner, D. (1993) Characterization of a Paracoccus denitrificans mutant pleiotropically impairs in nitrogen metabolism. Arch. Microbiol. 160, 166-168.

1991:


Ahmadian, M.R., Kreuzer, R. and Sprinzl, M. (1991) Overproduction of the Thermus thermophilus elongation factor Tu in Eschericia coli. Biochimie 73, 1037-1043.

Ghozlan, H.A., Ahmadian, M.R., Fröhlich, M., Sabry, S. and Kleiner, D. (1991) Genetic tools for Paracoccus denitrificans. FEMS Microbiol. Lett. 82, 303-306.


1990:

Weißhaar, M., Ahmadian, M.R., Sprinzl, M., Satoh, M., Kushiro, A. and Tomita, K. (1990) Sequences of four tRNA genes adjacent to the tuf2 gene of Thermus thermophilus. Nuc. Acids Res. 18, 1902.