Cardiac Tissue Engineering with Automated Bioreactor Systems

Tissue engineering, the artificial creation of biological tissue through targeted cell cultivation in matrix scaffolds, is a promising research approach in regenerative medicine and the production of patient-specific tissues.

In our group, a broad spectrum of tissue engineering methods is implemented from the decellularization of whole hearts through the repopulation of tissue scaffolds with new cells to the cultivation of tissues in a controlled physiological environment. Computer-assisted perfusion equipment and automated bioreactor systems are used to ensure controlled tissue processing. The success of the tissue engineering is examined and assessed with molecular biological and biochemical analysis methods using nucleic acid analyses, protein assays and histological staining.

Team

• Dr. rer. nat. Jessica Isabel Selig
• B. Sc. Caroline Küppers
• cand. med. Joana Boulgaropoulos
• Marieke Sophie Braß

Collaborators

• Prof. Dr. Karlheinz Preuß, Institut für Technische Mikrobiologie, Hochschule Mannheim

Publications

• Selig JI, Boulgaropoulos J, Niazy N, Ouwens DM, Preuss K, Horn P, Westenfeld R, Lichtenberg A, Akhyari P, Barth M (2021) Crosstalk of Diabetic Conditions with Static Versus Dynamic Flow Environment-Impact on Aortic Valve Remodeling. Int J Mol Sci. 22 (13). doi: 10.3390/ijms22136976
• Niazy N, Barth M, Selig JI, Feichtner S, Shakiba B, Candan A, Albert A, Preuss K, Lichtenberg A, Akhyari P (2021) Degeneration of Aortic Valves in a Bioreactor System with Pulsatile Flow. Biomedicines. 9 (5). doi: 10.3390/biomedicines9050462