Organoids as 3-dimensional cell culture sytems
Cell culture has been historically used to answer biological questions.
However, since cells growing on a petri dish are lacking the proper architecture, they would feature in a complex organism, the questions that shall be answered at present are becoming too complex for the classical 2D cell culture model. Here the contact to other cells is reduced to a minimum and issues like cell-cell contact, cell-cell communication, as well as the organization of multiple different cell types and their interactions cannot be adequately mimicked.
To overcome these limitations we establish three-dimensional immune cell containing organoids.
The 3D-cell culture is performed in a 96-well plate format and is easy accomplished, offering the potential for high throughput experiments.
From an ethical point of view this model can be even an alternative to in vivo experiments and will help to reduce numbers of required animal experiments.
The formation of adipose and cardioid organoids will potentially help in the future to answer questions regarding cardial immunity, obesity, diabetes and many more pathological conditions connected to cardiac and metabolic related diseases.
- Dr. rer. nat. Elvira Weber
- M.Sc. Melanie Cappallo
- B.Sc. Roya Batool
- cand. med. Miral Hayungs
- cand. med. Lotte A. Görtz