AG Sergeeva: Molecular Neurophysiology
We characterize the functional genome of identified brain neurons using cellular electrophysiology. We study aminergic neurons controlling wakefulness with a focus on histaminergic and catecholaminergic neurons. Comprehensive knowledge on their pharmacological and electrophysiological features, molecular heterogeneity and connectivity will improve the therapy of sleep disorders and strategies for general anesthesia and lead to the development of wake-promoting therapies for narcolepsy, morbus Parkinson and hepatic encephalopathy. We use reporter mouse lines and mouse models of human diseases. Our methods are electrophysiology (patch-clamp) from brain slices, cultures and acutely isolated neurons, microelectrode-array (MEA) recordings, single-cell RT-PCR, Real-time PCR, immunohistochemistry and intracellular calcium imaging. We are partially allocated to the Institute of Neural- and Sensory Physiology.
Olga A. Sergeeva
Karolina Mazur (cand. rer. nat.)
Anthony Nucaro (cand. med.)